Main Page

Welcome to The Lu Lab Wiki!

The essential survival guide in the Lu Lab. Currently expanding. To access, please e-mail the administrator (Tsaiyilu).

House Rules

• The Lu Lab Research Philosophy (v1.0 as of 10/22/24).
• The Lu Lab Manual/Onboarding information (In Progress).
• Lab Duty
• Cell Culture Room - Rules of Use (10/8/2025)
• Dr. David Ho's advice to young scientists (6/14/2014 in Cambridge, MA) (public)

Protocols

• Mouse survival surgery
• Intracardiac perfusion
• Immunohistochemistry/Immunocytochemistry/In situ hybridization
• OPC isolation and cultures
• Acute brain slice preparation
• Mouse demyelination models
• Developing brain fixation
• In vitro Ca²⁺ imaging (by Fluo-4 dye)
• Sample Preparation for ATAC-Seq

Recipes

• Recipes for OPC primary cell cultures
• Quick and dirty gDNA isolation
• Recipes for Physiology
• Recipes for immunohistochemistry/immunocytochemistry
• Recipes for injectable drugs
• Recipes for Flow Cytometry

Transgenic Mouse Lines

• Mouse naming system
• OPC lines
• Oligodendrocyte lines
• Neuronal lines
• Reporters
• Floxed lines and others

Data Management and Other Resources

• General principle when saving your electronic data
• Mouse colony management
• Recommended Software/package for data analysis and publication
• Shared lab accounts/licenses
• Useful online resources
• Useful web tools